An endochitinase gene (chit33-cDNA) from the biocontrol fungus Trichoderma atroviride was overexpressed under the CaMV35S constitutive promoter in 2 Dining Room Chairs canola (R line Hyola 308).Transformation of cotyledonary petioles was achieved via Agrobacterium tumefaciens.The insertion of the transgene was verified by PCR and Southern blotting.
Factors Associated with Caregiver Sleep Quality Related to Children with Rare Epilepsy Syndromes
Objective: To evaluate the impact of pediatric sleep disturbances and night-time seizure monitoring of children with rare epilepsy syndromes on the sleep quality and mental health of caregivers.Study design: A cross-sectional study was conducted using caregiver entered data from the Rare Epilepsy Network on MEDI C PLUS W/MG pediatric sleep disturba
Vertical Force Estimation of Heavy-Loaded Radial Tire Based on Circumferential Strain Analysis
In order to realize the quantitative estimation of tire vertical force, the algorithm of heavy-loaded tire vertical force estimation based on circumferential strain analysis is studied.A finite element analysis model of 16.00R20 tire is established.A comparison of tire loading test and modal vibration test indicates that the vertical stiffness erro